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human breast cancer tissue array  (Novus Biologicals)


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    Novus Biologicals human breast cancer tissue array
    Human Breast Cancer Tissue Array, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+tissue/Human+Breast+Tissue+MicroArray+(Cancer)/pmc12478062-532-1-9
    Average 93 stars, based on 22 article reviews
    human breast cancer tissue array - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Formalin-fixed Paraffin-Embedded:

    Article Title: Lysyl oxidase-like 2 promotes migration in noninvasive breast cancer cells but not in normal breast epithelial cells.
    Article Snippet: .. A formalin-fixed, paraffin-embedded histoarray slide containing human tissue sections from various cancers and normal adjacent tissues, including 1 normal human breast tissue sample and 1 infiltrating ductal carcinoma tissue sample, were obtained from Imgenex (San Diego, CA). .. Sections were deparaffinized, rehydrated, blocked with 5% normal goat serum (NGS; Pierce; Rockford, IL) for 30 min, then incubated with either the LOXL2 antibody (1:300, Fong et al, 2007) or rabbit IgG (4 lg/ml; Zymed; negative control) in 1% NGS for 2 hr, followed by Alexa Fluor 488-labeled goat anti-rabbit IgG secondary antibody (1:200; Molecular Probes) in 1% NGS for 45 min.

    Immunohistochemistry:

    Article Title: Enhanced ATRA-related compounds for the treatment of proliferative diseases, autoimmune diseases, and addiction conditions
    Article Snippet: .. Immunohistochemistry Analyses on Tissue Microarrays Formalin-fixed and paraffin-embedded tissue microarrays of human breast tissue were purchased from Imgenex (IMH-364 and 371). .. Rab2A (Proteintech Group) and ALDH1 (BD biosciences) staining was performed following the manufacturer's protocol Immunolabeling was visualized with a mixture of DAB solution (Vector Laboratories), followed by counterstaining with hematoxylin.

    Immunohistochemical staining:

    Article Title: Transforming growth factor-beta1 regulation of ATF-3 and identification of ATF-3 target genes in breast cancer cells.
    Article Snippet: Transforming growth factor-b1 (TGF-b1) is a crucial molecule for stimulation of breast cancer invasion and formation of bone metastases.. The molecular mechanisms of how TGF-b1 mediates these effects have yet to be completely determined.. We have found that activating transcription factor-3 (ATF-3) is strongly stimulated and its level is sustained by TGF-b1 in highly invasive and metastatic human breast cancer (MDA-MB231) and in mouse mammary pad tumor cells (r3T).

    Staining:

    Article Title: Transforming growth factor-beta1 regulation of ATF-3 and identification of ATF-3 target genes in breast cancer cells.
    Article Snippet: Transforming growth factor-b1 (TGF-b1) is a crucial molecule for stimulation of breast cancer invasion and formation of bone metastases.. The molecular mechanisms of how TGF-b1 mediates these effects have yet to be completely determined.. We have found that activating transcription factor-3 (ATF-3) is strongly stimulated and its level is sustained by TGF-b1 in highly invasive and metastatic human breast cancer (MDA-MB231) and in mouse mammary pad tumor cells (r3T).



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    Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published <t>microarray</t> data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.
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    Image Search Results


    Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

    Journal: iScience

    Article Title: Promotion of breast cancer by the DNPH1 enzyme

    doi: 10.1016/j.isci.2026.115227

    Figure Lengend Snippet: Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

    Article Snippet: Human breast tissue microarray , ISU Abxis , A312.

    Techniques: Over Expression, Expressing, Microarray, Standard Deviation, Derivative Assay, Western Blot, Immunohistochemical staining, Staining, Immunohistochemistry, Two Tailed Test

    ARTS is predominantly expressed in resistant breast cancer tissues and promotes chemoresistance in breast cancer cells. (A) Screening workflow for chemoresistance-related genes in breast cancer. DEGs from GSE288073 are shown in a volcano plot, followed by overlap with apoptosis-related and mitochondria-related gene sets, yielding 12 DEGs; ARTS was prioritized and evaluated by Kaplan–Meier survival analysis for postchemotherapy prognosis. FPKM, fragments per kilobase of transcript per million mapped reads. (B) Representative hematoxylin and eosin (H&E) and IHC images of ARTS in paired pre- and post-NAC samples from NAC-sensitive and NAC-resistant patients. The black arrow indicates a residual small cluster of tumor cells. Scale bar, 50 μm. (C) Association of ARTS protein with MPG score and Ki-67 in pre- and post-NAC samples. (D) Kaplan–Meier analyses of OS and RFS stratified by ARTS protein (low versus high). (E and F) MTT and (G and H) colony formation assays in sh-Ctrl versus sh-ARTS MDA-MB-231 and Flag versus Flag–ARTS MCF-7 cells under the indicated treatments. * P < 0.05; *** P < 0.001. ns, not significant.

    Journal: Research

    Article Title: ARTS Confers Chemoresistance of Breast Cancer by Inducing Apoptosis-Dependent Autophagy via Livin–MDM2–p53 Pathway

    doi: 10.34133/research.1086

    Figure Lengend Snippet: ARTS is predominantly expressed in resistant breast cancer tissues and promotes chemoresistance in breast cancer cells. (A) Screening workflow for chemoresistance-related genes in breast cancer. DEGs from GSE288073 are shown in a volcano plot, followed by overlap with apoptosis-related and mitochondria-related gene sets, yielding 12 DEGs; ARTS was prioritized and evaluated by Kaplan–Meier survival analysis for postchemotherapy prognosis. FPKM, fragments per kilobase of transcript per million mapped reads. (B) Representative hematoxylin and eosin (H&E) and IHC images of ARTS in paired pre- and post-NAC samples from NAC-sensitive and NAC-resistant patients. The black arrow indicates a residual small cluster of tumor cells. Scale bar, 50 μm. (C) Association of ARTS protein with MPG score and Ki-67 in pre- and post-NAC samples. (D) Kaplan–Meier analyses of OS and RFS stratified by ARTS protein (low versus high). (E and F) MTT and (G and H) colony formation assays in sh-Ctrl versus sh-ARTS MDA-MB-231 and Flag versus Flag–ARTS MCF-7 cells under the indicated treatments. * P < 0.05; *** P < 0.001. ns, not significant.

    Article Snippet: Human breast cancer tissue samples were obtained from the First Affiliated Hospital of Anhui Medical University (Hefei, Anhui, China).

    Techniques: